Chemical Industry Press Carle A. Celtic.
database and cyber source Appendix 6 poultry database and cyber source Appendix 7 fish
Transgenic animal technology and functional gene into animal technique is a powerful tool for the analysis of complex biological processes, which solves the problem across the biomedical, biological applications and agricultural production areas of science.The first edition of the Handbook of original is the world's first book written by the world famous Laboratory Animal Transgenic Technology Handbook, illustrated introduction to a variety of important laboratory animal and domestic animal species gene transfer technology, this method is the leading global laboratory method.This manual for the second edition, new edition to the first edition was updated content increased 80%, widely used in animal model systems in gene engineering techniques such as microinjection, ES cell nuclear transfer.In order to enable readers to get more information and convenience in research, this manual also comprehensively provides database in the transgenic animal technology and cyber source, and naming of the transgenic animal is described in detail.The handbook can be used as a desk guide engaged in biotechnology research animal subjects, at the same time as the technical staff, senior undergraduate and postgraduate training books.
The first part summarizes the application of 1 transgenic animal technology introduction 1.1 1.2 1.3 book, history and overview of reference second part of the transgenic animal model in mice to produce 2 DNA microinjection and transgenic animal production 2.1 introduction < br>2.2 general method of 2.3 summary thanks reference appendix 3 effects of transgenic animal production 3.1 introduction 3.2 preparation 3.3 troubleshooting 3.4 transgenic phenomenological 3.5 summary Acknowledgements References 4 embryonic stem cell gene targeting: 1. 4.1 history and method of embryo stem cells of embryonic stem cell gene targeting 4.2 4.3 method for reference 5 embryonic stem cell gene targeting: a brief history of 5.3 Cre/ loxP II. Systematic review, establishment and test of 5.4 Cre/loxP and 5.5 5.6 transgenic model method summary Acknowledgements References 6 retrovirus mediated gene transfer of 6.1 introduction and discussion of 6.2 thanks reference 7 nuclear transplantation < br>7.1 conditional 5.1 introduction 5.2 conditional modelIntroduction and discussion of 7.2 materials and equipment of 7.3 micro tool preparation 7.4 preimplantation SCNT 7.5 embryonic stem cells and somatic cell nuclear transfer Acknowledgements References third part of the experiment and domestic transgenic species 8 transgenic rats, 8.2 rats 8.1 the importance of 8.3 transgenic rats effect of 8.4 make 8.5 conclusion reference 9 transgenic rabbit production 9.1 transgenic rabbits by application of 9.2 transgenic experiment of rabbit embryo manipulation of 9.3 material and method references 10 transgenic fish production of 10.1 10.2, discuss 10.3 gene transfer methods 10.4 transgenic lineages of the establishment and maintenance of 10.5 transgenic fish care and contain 10.6 of transgenic fish future Acknowledgements References 11 transgenic poultry production 11.1 introduction and discussion of 11.2 11.3 summary of reference 12 by DNA microinjection transgenic porcine 12.1 introduction and discussion of the 12.2 method of 12.3 transgenic pigs in addition method 12.4 summary reference 13By DNA microinjection to generate transgenic animal 13.1 introduction 13.2 for microinjection zygotes produced 13.3 ruminant animal zygote microinjection of 13.4 microinjected embryos transplant 13.5 application fields and prospects of Acknowledgements References 14 transgenic non-human primates, and discuss the method of making 14.1 14.2 14.3 summary acknowledgment reference 15 cattle, sheep and porcine nuclear transfer technique, 15.2 15.1 15.3 summed up the general method for reference fourth part of molecular biology, analysis and implementation of 16 transgenic expression vector 16.1 introduction 16.2 transcription region of 16.3 promoter 16.4 boundary zone 16.5 conclusion and Prospect of Acknowledgements References 17 transgene integration analysis of 17.1, 17.2 transgenic detection of < br>17.3 transgenic evaluation parameters of 17.4 transgenic analysis of 17.5 transgenic lineages established 17.6 homozygous mice derived 17.7 summary reference 18 transgene integration detection method for optimizing 18.1 18.2 PCR, 18.3 and Kao Wen discussion summary
Database and cyber source index
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